Capping of exogenous Forssman glycolipid on cells

Capping of Exogenous Forssman

Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution

Forssman antigen, a neutral glycosphingolipid carrying five monosaccharides, was localized in epithelial MDCK cells by the immunogold technique. Labeling with a well defined mAb and protein A-gold after freeze-substitution and low temperature embedding in Lowicryl HM20 of aldehyde-fixed and cryoprotected cells, resulted in high levels of specific labeling and excellent retention of cellular ult...

Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: its possible identity with "A-like antigen" in human cancer.

The heterogenetic Forssman antigen is a glycosphingolipid, a ceramide pentasaccharide with the structure GalNAcalpha1-->3GalNAcbeta1-->3Galalpha1-->4Galbeta1-->4Glc-->ceramide. Forssman-positive animals are capable of synthesizing this compound in tissues or in erythrocytes, in contrast to the Forssman-negative species, including humans, which are incapable of adding the last carbohydrate in th...

Reaction of antibodies that cause paroxysmal cold hemoglobinuria (PCH) with globoside and Forssman glycosphingolipids.

Many antibodies that cause paroxysmal cold hemoglobinuria (Donath-Landstelner antibodies) appear to be directed against the blood group P antigen. We recently Identified this antigen as the glycosphingolipld globoside GaINAc(fl, 1-’3)Gal(a, 1-’4)Gal(fl, 1-’4)GlcCer, and we examined the reaction of four Donath-Landsteiner antibodies with anti-P specificity, and an anti-P acId autoagglutinin, wit...

Reaction of Antibodies That Cause Paroxysmal Cold Hemoglobinuria ( PCH ) With Globoside and

Many antibodies that cause paroxysmal cold hemoglobinuria (Donath-Landstelner antibodies) appear to be directed against the blood group P antigen. We recently Identified this antigen as the glycosphingolipld globoside GaINAc(fl, 1-’3)Gal(a, 1-’4)Gal(fl, 1-’4)GlcCer, and we examined the reaction of four Donath-Landsteiner antibodies with anti-P specificity, and an anti-P acId autoagglutinin, wit...